Effects of different extenders on preservation of Jamunapari buck semen

Abstract

The aim of this study was to investigate the effects of different extenders on the quality of Jamunapari buck semen. Semen was collected from three adult Jamunapari bucks by Artificial Vagina (AV) method once a week. Immediate after collection, fresh semen was evaluated for recording its quality including volume, color, density, concentration, mass motility, pH and membrane potentiality. Each semen sample was divided into two groups; one for chill and another for cryo-preservation. Three different types of semen extenders; Tris (T), Skim milk (S) and Skim milk D-glucose (SD) were added in each group of semen dividing into three parts and then preserved accordingly. Chilled semen was evaluated on day 2 and day 4 of chilling at refrigerator (4ºC) and frozen semen on day 5 and day 12 of cryopreservation at liquid nitrogen (LN2). Preserved semen was evaluated for motility, viability, membrane potentiality (HOS test) and morphology. Average volume, mass motility, and sperm concentration were 1.22±0.26 ml, 4.01±0.67and 3.13±0.27×109 /ml, respectively. The motility, viability, normal morphology and membrane potentiality and pH were 86.67.5±3.00, 82.13±2.18, and 96.52±1.69, 83.2±3.20, and 6.74±0.28, respectively. The quality of chill semen was the best in Tris-based extender (P<0.05) followed by Skim milk D-glucose based extender and skim milk-based extender. Frozen semen quality was also maintained at a higher level (P≤0.05) in Tris-based extenders compared with the other two extenders studied. It was concluded that a Tris-based semen extender was the best choice for the preservation of Jamunapari buck semen in comparison with Skim milk D-glucose and skim milk-based extender.