The present study was conducted to assess the semen quality and sperm biokinetic parameters of Red Junglefowl (RJF) available in Bangladesh. A total of 12 RJF cocks were primarily selected and trained, and finally, 6 cocks were proven suitable for semen c

Abstract

The present study was conducted to assess the semen quality and sperm biokinetic parameters of Red Junglefowl (RJF) available in Bangladesh. A total of 12 RJF cocks were primarily selected and trained, and finally, 6 cocks were proven suitable for semen collection. Experimental birds were maintained under captive conditions with similar and uniform management. Semen was collected twice a week by the dorso-abdominal massage method and used for the evaluation of pH and concentration of ejaculated fresh semen. Total motility, progressive motility and biokinetic parameters such as curvilinear velocity (VCL), average path velocity (VAP), straight-line velocity (VSL), linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were analyzed by a Computer Assisted Semen Analyzer (CASA). Average volume, pH and sperm concentration of the ejaculated semen of six (6) different proven cocks ranged from 0.35–0.50 ml, 7.0–7.22 and 1.70–3.50 ×109/ml, respectively. Total motility and progressive motility were 91–99% and 57–62%, respectively. Among the sperm biokinetic parameters, the highest VCL, VAP, VSL, and STR were 105.50 μm/sec, 68.24 μm/sec, 29.23 μm/sec and 58.77%, respectively. The results of the present study revealed that the quality of semen varies from cock to cock but the average results showed that almost all of the cocks produced good quality semen with standard total motility, progressive motility and biokinetic parameters. Thus, it may be concluded that RJF semen can be collected effectively by abdominal massage method which yields reasonable production in terms of quality and quantity for semen storage and may therefore be used for artificial insemination to increase their population.